Abstract
The objective of this study was to determine the effect of phloroglucinol on in vitro rooting of sugarcane cultivar C90-469. A completely randomized experimental design was used with seven treatments, three concentrations of phloroglucinol (10, 15 and 20 mg L−1) in combination or not with 1.3 mg L−1 of indole-3-acetic acid (IAA) and as control the liquid culture medium without phloroglucinol. After 15 days of culture, the number of shoots rooted, height, number of leaves, fresh weight of the in vitro plant (gFW), number of roots, length of the main root and the total chlorophyll content (SPAD units) were evaluated. In the ex vitro acclimatization phase, the survival of the in vitro plants was evaluated 15 days after transplanting. Concentration of 20 mg L−1 of phloroglucinol combined with 1.3 mg L−1 of IAA and 40 g L−1 of sucrose had 100% rooting. A greater number of roots per plant, the formation of root hairs and a high survival percentage (87.8%) of the in vitro plants under ex vitro acclimatization conditions in a shade house were recorded.
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