Effect of phleomycin on the replication of mengovirus in novikoff hepatoma cells.

Abstract

The replication of mengovirus was studied in the presence and absence of phleomycin. When the antibiotic was added simultaneously with virus infection, an exponential decrease in the yield of virus was observed with increasing concentrations of phleomycin. The induction of viral RNA polymerase activity was inhibited almost completely by 20 and 40 μg of phleomycin per ml. The antibiotic at the same concentrations gave 93 and 97% inhibition of protein synthesis and 96.5 and 98.6% inhibition, respectively, of RNA synthesis in infected cells.