Abstract
1. 1. Effects of pH, pCO 2 and bicarbonate on metabolism of [ 14C 6]glucose in the isolated perfused rat heart were studied. 2. 2. With an alkaline extracellular pH, glucose uptake, lactate production and glycogen breakdown increased, and oxidation of exogenous glucose remained constant. These findings are compatible with enhancement of phosphofructokinase (EC 2.7.1.11) activity. 3. 3. At an acid extracellular pH, glucose uptake and lactate production decreased, glycogen levels were unchanged, and 14CO 2 production was unchanged until pH fell below 7.0, at which time 14CO 2 decreased. 4. 4. Glucose uptake, lactate production, 14CO 2 production and glycogen levels were similar in hearts perfused at pH 7.50 with either a bicarbonate buffer or 30 mM Tris.
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