Abstract
The effect of pH on the electroactivity of horse heart cytochrome c has been studied by differential pulse polarography, differential pulse voltammetry at the gold electrode, cyclic voltammetry and spectrophotometry; the gold electrode has been activated by 4,4′-bipyridine. The experimental results have been interpreted on the basis of the existence of a pH-dependent equilibrium between two electroactive cytochrome c forms; in agreement with the Lambeth et al.'s scheme the existence of transient species can be postulated. From spectrophotometric and differential pulse voltammetric experiments the p K a value of 8.1 ± 0.1 has been calculated for the equilibrium between the neutral and alkaline cytochrome c forms: state III ⇌ estate IV + H +.
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