Effect of PG0839 gene of Porphyromonas gingivalis on inflammatory cytokine in human oral epidermoid carcinoma KB cell

Abstract

To investigate the effect of PG0839 gene form Porphyromonas gingivalis (Pg) on inflammatory cytokine expression in human oral epidermoid carcinoma KB cell. A mutant in the PG0839 gene of Pg was created by insertional inactivation. Group 1 was chanllenged with PgW83 strain, group 2 with PG0839-defective mutant, and the control group with Dulbecco's modified Eagle's medium only. KB cells were co-cultured with presence of bacteria for 24 h. At the time point of 0.5, 2, 6, 12 and 24 h, cells were stored in Trizol. The mRNA expression of interleukin-1β (IL-1β) and Toll like recepector-4 (TLR-4) was examined by reverse transcription polymerase chain reaction. At 2 h and 6 h, IL-1β mRNA expression was lower in group 2 than in group 1 (2 h: 0.31 ± 0.11 versus 0.95 ± 0.48, P < 0.05; 6 h: 0.57 ± 0.20 versus 1.29 ± 0.55, P < 0.05). At 0.5 h and 6 h, TLR-4 mRNA expression was lower in group 2 than in group 1 (0.5 h: 0.20 ± 0.09 versus 0.58 ± 0.09, P < 0.05; 6 h: 0.34 ± 0.04 versus 0.71 ± 0.18, P < 0.05). PG0839 gene may play an important role in Pg-induced inflammatory effects of KB cell.