Effect of PFOS and PFOA Exposure in Utero on Reproductive Hormones Levels at Birth

Abstract

Effect of PFOS and PFOA Exposure in Utero on Reproductive Hormones Levels at BirthAbstract Number:2403 Sachiko Itoh*, Atsuko Araki, Chihiro Miyashita, Hiroyuki Nakazawa, Takahiko Mitsui, Kazutoshi Cho, Seiko Sasaki, Tamiko Ikeno, Katsuya Nonomura, and Reiko Kishi Sachiko Itoh* Hokkaido University Centre for Environmental and Health Sciences, Japan, E-mail Address: [email protected] Search for more papers by this author , Atsuko Araki Hokkaido University Centre for Environmental and Health Sciences, Japan, E-mail Address: [email protected] Search for more papers by this author , Chihiro Miyashita Hokkaido University Centre for Environmental and Health Sciences, Japan, E-mail Address: [email protected] Search for more papers by this author , Hiroyuki Nakazawa Hoshi University School of Pharmacy and Pharmaceutical Sciences, Department of Analytical Chemistry,, Japan, E-mail Address: [email protected] Search for more papers by this author , Takahiko Mitsui Hokkaido University Graduate School of Medicine, Department of Urology, Japan, E-mail Address: [email protected] Search for more papers by this author , Kazutoshi Cho Hokkaido University Graduate School of Medicine, Department of Obstetrics,, Japan, E-mail Address: [email protected] Search for more papers by this author , Seiko Sasaki Hokkaido University Graduate School of Medicine, Department of Public Health Sciences, Japan, E-mail Address: [email protected] Search for more papers by this author , Tamiko Ikeno Hokkaido University Centre for Environmental and Health Sciences, Japan, E-mail Address: [email protected] Search for more papers by this author , Katsuya Nonomura Hokkaido University Graduate School of Medicine, Department of Urology, Japan, E-mail Address: [email protected] Search for more papers by this author , and Reiko Kishi Hokkaido University Centre for Environmental and Health Sciences, Japan, E-mail Address: [email protected] Search for more papers by this author AbstractPerfluorooctanesulfonate (PFOS) and Perfluorooctanoate (PFOA) are used in variety of industrial products and have been alleged persistent organic pollutants due to the chemical stability. However, there was no epidemiological report addressing the effects of prenatal exposure to PFOS/PFOA on human reproductive hormones at birth. Aim of this study was to examine the association of the concentrations of PFOS and PFOA in maternal serum with reproductive hormones in cord blood. 514 women who took prenatal health care at the Toho hospital (Sapporo, Hokkaido, Japan) were selected to participate in this study. We obtained the perinatal information from the questionnaire and medical records. PFOS and PFOA concentrations in maternal serum were measured after the second trimester of pregnancy or after delivery by LC/MS/MS, and concentrations of estradiol, total testosterone, progesterone(P4), inhibin-B, Insulin-like factor 3 (INSL3), sex hormone-binding glogulin (SHBG), prolactin (PRL), follicle-stimulating hormone, and luteinizing hormone were measured from cord blood. The univariate and multiple linear regression analyses were performed to investigate the relationships between PFOS and PFOA levels and these hormones.Among 514 women, 257 mothers and their infants who had both data of PFOS/PFOA levels and reproductive hormones were analyzed. In adjusted regression models, the concentration levels of PFOS in maternal serum showed negative association statistically with PRL, Inhibin-B in male infants, and P4, SHBG, PRL in female infants. Maternal PFOA levels associated positively with PRL in male infants, while PFOA was not related significantly with any hormones in female infants. This study showed that there was significant association between maternal PFOS/PFOA exposure at low level and infant reproductive hormone levels. These results also indicate the possibility that PFOS/PFOA may disrupt infants’ reproductive hormone homeostasis through placental transfer of PFOS/PFOA.