Effect of petroleum wastewater treated with gravity separation and magnetite nanoparticles adsorption methods on the blood biochemical response of mrigal fish (Cirrhinus cirrhosus).

Abstract

Drainage of treated wastewater to surface water is a severe threat to the health of aquatic organisms. This study aimed to evaluate the effects of 0.5 and 1% water-soluble fractions of crude oil (WSFO), WSFO treated with magnetic nanoparticles of Fe3O4 (TWSFO-Fe3O4) and with the gravity separation method (TWSFO-GSM) on Cirrhinus cirrhosis for 21 days. The rate of erythrocyte hemolysis in fish exposed to untreated 0.5 and 1% WSFO were significantly high. The activities of alanine aminotransferase (ALT), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP) were significantly increased in the groups exposed to TWSFO-GSM compared to the control group, while lactate dehydrogenase (LDH) was reduced. No significant differences in LDH, ALT, ALP, and GGT activities were observed in the fish treated with TWSFO-Fe3O4. The aspartate aminotransferase activity was significantly increased after exposure to TWSFO-Fe3O4 (1%) and TWSFO-GSM. The levels of triglyceride were decreased, whereas glucose, cholesterol, and cholinesterase activity increased in fish after both treatments. The total protein and albumin contents significantly decreased in fish under exposure to both doses of TWSFO-Fe3O4 and TWSFO-GSM. The globulin level decreased in fish exposed to TWSFO-Fe3O4 (1%) and TWSFO-GSM. Glutathione peroxidase, catalase, glucose-6-phosphate dehydrogenase activities, and total antioxidant levels were significantly reduced in the hepatocytes of fish exposed to TWSFO-Fe3O4, TWSFO-GSM, and WSFO, while superoxide dismutase activity and malondialdehyde content were increased. This study showed that despite removing oil drips from the WSFO, the xenobiotics present in the effluent treated by gravitational or nano-magnetite methods caused changes in biochemical parameters and induced oxidative stress. Therefore, it is recommended to prevent the discharge of treated effluent from the oil and petrochemical industries to aquatic ecosystems.