Abstract
The effect of crude oil on germination, growth and yield of broad bean was tested in contaminated soil. A series of soil pollution levels, ranged between 1% and 10% (w/w) were prepared. The seed germination, vegetative growth and yield of broad bean were investigated. Low concentration of crude oil was found to stimulate the vegetative growth and increase the yield. High concentration of crude oil (4% and above) reduced the germination percentage ,vegetative growth and yield. At concentrations of 8% & 10%, many seedlings had reduced growth and survival. Hydrocarbons accumulated in shoot and pods (seeds) were separated and monitored by quantitative gas chromatography (GC.). The residual n-alkanes and iso-alkanes of the saturates fraction were decreased with increasing the pollution levels. Lighter n-alkanes (C10 – C20) were found only in the shoots, while the higher carbon number n-alkanes (C20 – C31) were detected in both shoots and pods. The results suggested not to grow edible plants in areas subjected to low or high concentration of petroleum oil.
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