Abstract
The experiment under lab condition was conducted to investigate the effect of petroleum hydrocarbon contamination on soil microorganisms and biodegradation. The experiment was conducted in 12 treatment and three replication. The treatments include soil contaminated with Kerosene, Diesel, lubricate oil and used lubricate oil in three concentration 0%, 5%, and 10%. contamination with hydrocarbons significantly decreased the count of heterotrophic bacteria and there was reversal relationship between the contaminant concentration and the count of heterotrophic bacteria. The results indicated soil contamination with hydrocarbons significantly increased the count of Hydrocarbon Utilizing Bacteria (HUB). The effect of petroleum hydrocarbons on fungi count differed according to the type of hydrocarbons, concentration of contaminant and the periods of incubation. Our results show that soil contamination by hydrocarbons caused significant increase in actinomycetes count at all hydrocarbon types and concentrations except 5% used and non-used lubrication oil. The higher percentage of biodegradation in hydrocarbons contaminated soil 49.6% was recorded in soil contaminated with 5% kerosene while the lower percentag10.8% was recorded with soil contaminated 10% lubricate oil. .
Highlights
Considering the rapid growth in the world’s population, crude oil continues to be the main source of energy and raw material for industries worldwide (Abbasian et al, 2016)
Standard plate count methods were used to prepare nutrient agar (NA) for estimation of the heterotrophic bacteria, potato dextrose agar (PDA) for estimation of fungi, Starch casein nitrate (SCN) agar for estimation of actinomycetes, and Luria bertani agar (LBA) for estimation of hydrocarbon utilizing bacteria (HUB)
The results indicated soil contamination with hydrocarbons significantly decreased the count of heterotrophic bacteria and there was reversal relationship between the contaminant concentration and the count of heterotrophic bacteria
Summary
Considering the rapid growth in the world’s population, crude oil continues to be the main source of energy and raw material for industries worldwide (Abbasian et al, 2016). Standard plate count methods were used to prepare nutrient agar (NA) for estimation of the heterotrophic bacteria, potato dextrose agar (PDA) for estimation of fungi, Starch casein nitrate (SCN) agar for estimation of actinomycetes, and Luria bertani agar (LBA) ( in LBA yeast extract was replaced with kerosene, diesel or lubricate oil as carbon source) for estimation of hydrocarbon utilizing bacteria (HUB). Our results show that there were no significant differences in count of heterotrophic bacteria between soil contaminated with non-used and used lubricate oil Hydrocarbon Types After 4 weeks of incubation
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