Abstract
It has been widely studied and demonstrated that circadian rhythm, as well as circadian clock genes, plays an important role in many physiological and pathological processes. Period1 (Per1) gene is a core gene in the circadian clock system that is involved in cell differentiation and apoptosis. To verify the role of PER1 on the proliferation of normal cells, we chose NIH3T3 cells to have their PER1 up-regulated by transfecting a Per1 expression plasmid and determining their proliferation rates. Previous studies demonstrated that cell migration could be regulated by matrix metallopeptidase 2 (MMP2), and this MMP2 protein had a relation with the circadian system. To investigate the function of PER1 on tumor cell migration, Lewis lung carcinoma (LLC) cells were chosen to have their PER1 up-regulated, and the MMP2 level and the migration of LLC cells were tested. Our study showed that PER1 overexpression led to MMP2 down regulation in both NIH3T3 and CCL cells and inhibited the proliferation rate in NIH3T3. Migration of LLC cells could also be suppressed by the overexpression of PER1, probably by the down-regulation effect of PER1 on MMP2.
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