Abstract
At present, the global prevalence of peripheral arterial disease is increasing year by year, and it has become a worldwide disease. Studies have shown that transplanting endothelial progenitor cells (EPCs) into ischemic tissues can improve the tissue ischemia, thereby having a therapeutic effect on peripheral arterial diseases. This indicates that EPCs play a therapeutic effect in peripheral arterial disease. Recent studies have shown that peptidylarginine deiminase (PAD) is involved in the regulation of epigenetics and its inhibitor Cl-amidine can improve endothelium-dependent vasodilation and significantly reduce the formation of arterial thrombosis. It can also play a role in hematopoietic stem cells that share the same origin with EPCs. Therefore, we speculate that PAD4 may also have an effect on EPCs through a similar mechanism, thereby participating in the damage and repair of peripheral arterial disease. Therefore, we first detected the expression of PAD4 in EPCs of peripheral arterial disease and detected changes in the number and function of endothelial progenitor cells in peripheral blood after injecting the PAD4 inhibitor Cl-amidine into mice. A mouse model of lower limb ischemia was established to explore the effect of PAD4 on the function of EPCs in peripheral arterial disease. The results show that PAD4 is highly expressed in peripheral arterial diseases and the PAD4 inhibitor Cl-amidine can increase the number of EPCs and can treat peripheral arterial diseases by improving the proliferation, migration, and vascularization of EPCs.
Highlights
Peripheral artery disease is a common circulatory system disease caused by limb ischemia caused by arterial vascular stenosis
Separation and Detection of endothelial progenitor cells (EPCs). e peripheral blood of each group of mice was separated into EPCs according to the method described in Section 2.2, and MTT, Transwell, 3.2
0 Normal healthy Peripheral arterial disease (b) GSK3β and the AMD3100 group mouse peripheral blood mononuclear cell cultures detected more EPC colony forming unit (EPC-CFU), and the Clamidine group was more than the AMD3100 group, and the difference was statistically significant (P < 0.05), as shown in Figure 2(a). e cells glow red after ingesting Dil-ac-LDL, and the cells glow green when combined with FITC-UEA-1
Summary
Peripheral artery disease (peripheral artery disease) is a common circulatory system disease caused by limb ischemia caused by arterial vascular stenosis. The current global prevalence of peripheral artery disease is about 3–12% [1]. Low- and middleincome countries are the hardest hit areas for peripheral arterial disease (approximately 70% of the number of patients), and peripheral arterial disease has become a major worldwide disease. Fowkes et al [2] reported in 2010 that about 200 million people in the world were currently affected by peripheral arterial disease to varying degrees. As an important disease currently attracting attention, it is necessary to conduct active research on the pathogenic mechanism of peripheral vascular disease and corresponding treatment measures. Surgical treatments and medical treatments have been proposed, the existence of surgical risks and drug side effects makes it necessary to explore safer and effective treatment methods to avoid the shortcomings of current treatments
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