Abstract
Insulin response on normal blood vessel is a relaxation. However, insulin produces contraction in insulināresistant (IR) vessels. IR has been associated with equine laminitis. The objective of this study was to evaluate the effectiveness of two potential drugs (Losartan & Pentoxifylline) to counteract the responses of experimentally inducedāinsulin resistant equine digital vessels to insulin in vitro. Palmar digital arteries and veins (2ā³ long) were collected from lean horses (overnight fasting plasma insulin 65pmol/L and glucose 5.614mmol/L) immediately after euthanasia. Eight 3āmm wide rings were cut (4 arterial and 4 venous) and were set in ināvitro tissue baths for response studies. Two rings from each vessel type were made IR by incubating them with insulin at a concentration of 10ā5M for 30 min. The other rings were used as control. Then all these rings were contracted with phenylephrine 10ā6.5M and when it reached a plateau either losartan (10ā5M) or pentoxifylline (10ā5M) was added to both insulin resistant and non resistant (control) rings and allowed to stay in the bath for 5 minutes, before a single dose of insulin (10ā5M) was added to all rings. The response to insulin was monitored for another 30 minutes. Losartan preātreatment of IR vessels produced contraction to insulin. On the contrary, pentoxifylline caused relaxation suggesting it could be beneficial in the treatment of equine laminitis. The study was supported by USDA 1433 Formula ā LSUāSVM.
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