Effect of penehyclidine on small intestinal motility in mice and rats

Abstract

Objective To investigate the effect of penehyclidine on small intestinal propulsion in mice and plasma level of motilin in rats.Methods The experiment was performed in 2 parts. In experiment Ⅰ , twenty-four healthy Kunming mice of both sexes weighing 17-22 g were randomly divided into 3 groups (n = 8 each): group control (C1), group Ⅱ received intraperitoneal (IP) atropine 0.3 mg/kg (A1) and group Ⅲ received IP penehyclidine 0.3 mg/kg (P1). Nutritional semi-solid cataplasm 0.8 ml was given into the stomach at 15 min after IP injection. The mice were killed 20 min later. The abdomen was cut open. The length of the small intestine and the distance between the pylorus and the nutritional semi-solid cataplasm in the small intestine were measured. The rate of intestinal propulsion was calculated. In experiment Ⅱ , twenty-four Wistar rats of both sexes weighing 170-220 g were randomly divided into 3 groups (n=8 each);group Ⅰ control (C2);group Ⅱ received IP atropine 2 mg/kg (A2) and groupⅢ received IP penchyclidine 2 mg/kg (P2). Blood samples were taken at 30 min after IP drug administration for determination of plasma motilin concentration (by radio-immuno-aasay). Results The rate of small intestinal propulsion was significantly lower in group A1 than in group C1. There was no significant difference in the intestinal propulsion between group C1 and P1. The plasma concentration of motilin was significantly lower in group A2 than in group C2 but there was no difference in plasma motilin concentration between group P2 and C2. The plasma concentration of motilin was significantly lower in group A2 than in group P2. Conclusion Penehyclidine has no effect on small intestine motility. Key words: Quinuclidines; Gastrointestinal motility; Intestine, small