Abstract
Staphylococcus enterotoxin B (SEB) is produced by the bacterium Staphylococcus aureus. SEB is the most common cause of food poisoning often accompanied with nausea, diarrhea, and intestinal cramping. The purpose of this study is to further investigate human peripheral blood mononuclear cells (PBMCs) in order to better understand the apoptosis related events induced by SEB. C‐Jun N‐Terminal Kinase (JNK) has been previously identified to be induced by SEB, and has demonstrated that it inter‐connects multiple signaling cascades. As a crucial pathway inter‐connector in SEB‐induced human PBMCs, we believe that JNK may possess inhibitory effects of SEB‐induced apoptosis. We will further evaluate the utilization of the intrinsic (caspase 3, MCLR1, BAX, etc.), extrinsic (caspase 8, caspase 10) pathways, and gene expression profile using RT and PCR. Protein expression patterns of some genes will be examined through ELISA. We believe that our work will allow us to better understand the complex interactions of multiple signal transduction pathways induced by SEB.
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