Abstract
Effect of Pathway‐Interconnectors in SEB‐Induced Apoptosis Related Events in Human PBMCs Staphylococcus enterotoxin B (SEB) is produced by the bacterium Staphylococcus aureus. SEB is the most common cause of food poisoning often accompanied with nausea, diarrhea, and intestinal cramping. The purpose of this study is to further investigate human peripheral blood mononuclear cells (PBMCs) in order to better understand the apoptosis related events induced by SEB. C‐Jun N‐Terminal Kinase (JNK) has been previously identified to be induced by SEB, and has known to inter‐connect multiple signaling pathways. As a crucial pathway inter‐connector in SEB‐induced human PBMCs, we believe that JNK may possess inhibitory effects of SEB‐induced apoptosis. Here we will attempt to evaluate the type of pathway (intrinsic [caspase 3, MCLR1, BAX, etc.], extrinsic [caspase 8, caspase 10] utilized by SEB by studying the gene expression profile using RT‐PCR. We will also investigate the Protein expression patterns of some genes through ELISA. We believe that our work will allow us to better understand the complex interactions of multiple signal transduction pathways induced by SEB.
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