Abstract
ABSTRACT Experiments were conducted to determine the effect of sample size and particle size on lipid oxidation of milled flaxseed. An initial experiment showed that most of the lipid oxidation detected in milled flaxseed samples occurred on the surface that was exposed to the air during storage. In a subsequent experiment, milled flaxseed at 100, 200 and 300 g were stored in the dark in rectangular pans for 8, 24 and 48 days at 50C. Lipid oxidation, as determined by surface lipid content, peroxide value and α-linolenic acid content, was lower in the 300 g samples than in the 100 g samples of milled flaxseed. Large versus small samples had less apparent lipid oxidation because of their inherently lower surface to volume ratio. In a third experiment, oxidation was lower in lipid extracted from coarse (>600 µm) than fine particles (<600 µm), after 48 days of storage. PRACTICAL APPLICATIONS This research has practical applications to the flaxseed milling and food industries. These results show that assessment of lipid oxidation in milled flaxseed needs to consider sample size (collection). Milled flaxseed oxidized predominantly at the sample surface where a polymerized layer of flaxseed particles formed over time. Relatively little oxidation occurred in the interior of the sample. Thus, sampling from the surface or interior of a milled flaxseed sample will end in different results and conclusions. Similarly, results will vary with surface : interior ratio of milled flaxseed. Food manufacturers may want to discard the top layer of milled flaxseed that has been exposed to air. Lipid oxidation was less with coarse than fine particles. Thus it would be prudent not to use milled flaxseed with a finer particle size than is necessary for acceptable end-use quality.
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