Abstract

 
 
 
 Purpose: To analyze the effect of enzyme papain administration, after its injection into abnormal scars of Rattus norvegicus rats, on pH alteration (ΔpH) of scar tissue, vascular endothelial growth factor (VEGF) expression levels, and determine their impact on collagen degradation.
 Methods: This study used Rattus norvegicus as an experimental animal. The pH measurement of the scar tissue was carried out before papain injection at 12th weeks while abnormal scar was established after papain injection at 15th weeks. Expression of vascular endothelial growth factor (VEGF) was examined by Western blot technique, while hydroxyproline levels were measured using QuickZyme Total Collagen Assay.
 Results: Changes in ΔpH, VEGF expression, and hydroxyproline levels in the treatment group were significant (p < 0.05) compared to control. Path analysis showed a direct relationship between the parameters (p < 0.01), except for the correlation between VEGF and hydroxyproline value (p = 0.23).
 Conclusion: Papain enzyme administration increased ΔpH value of abnormal scar tissue. A high dose of papain also causes an increase of collagen degradation process, as a response to angiogenesis deflation.
 
 
 
Highlights
Abnormal scarring consists of hypertrophic and keloid scars, resulting from the loss in balance of communication between cells
This study aims to analyze the effect of enzyme papain administration after injected into abnormal scars of Rattus norvegicus rats against pH alteration (ΔpH) of scar tissue, vascular endothelial growth factor (VEGF) expression levels, and determined their impact on collagen degradation
This process involves a phase of collagen synthesis that requires the formation of new blood vessels or neovascularization for the supply of oxygen and other functional cells to the wound site [4,16]
Summary
Abnormal scarring consists of hypertrophic and keloid scars, resulting from the loss in balance of communication between cells. Wound healing imbalance occurs in one of the three main phases to form the abnormal scar These are inflammation, proliferation, and remodeling [2]. Prolongation of inflammation will result in endothelial damage, due to the accumulation of pro-inflammatory cells and excessive fibroblast activity [3] This phase, evidenced by an increase in NF-κB levels, activates vascular endothelial growth factor (VEGF) to leading to an angiogenesis process, granulation tissue formation [4], and activation of fibroblasts [5,6].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
