Effect of Paclobutrazol concentration on initial formation stolon in potato plants by in vitro at different temperature conditions

Abstract

The increase in potato production is constrained condition in Indonesia grow in the area with an altitude of 1500m above sea level. It is known that at high temperatures the gibberellin acid (GA) content in plants will increase, thus inhibiting tuber formation. The use of growth retardants such as paclobutrazol can inhibit GA biosynthesis so that it can stimulate tuber formation. In this study, the aim of this study was to determine the effect of paclobutrazol concentration on the initial formation of potato plant stolons by in vitro applied at different temperatures. The seeding stage was carried out on potato explants that had been kept for 4 weeks under 16 h of radiation. Paclobutrazol was given by pouring it into an explant bottle and keeping it in dark conditions at different temperatures for 8 weeks. From the results of the study, it is known that the application of paclobutrazol concentration and different temperatures affect the formation of micro tubers. where at a concentration of 3 ppm (P1) paclobutrazol was able to inhibit stolon elongation, stimulating subapical stolon and tuber swelling. The interactions between the application of paclobutrazol concentrations and different temperatures affected subapical stolon and tuber swelling.