Effect of paclitaxol in rat orthotopic liver transplantation and its impact on the CTL

Abstract

Ojective To observation of the effect of paclitaxol in rat orthotopic liver transplantation and its impact on the cytotoxic T lymphocyte (CTL). Methods Wistar → SD rat liver transplantation model of acute rejection was established. The rats were divided into three groups: control group (blocking total hepatic blood flow), acute rejection group (Wistar → SD liver transplantation), paclitaxel group (Wistar → SD liver transplantation, paclitaxel intraperitoneal injection of 0.75 mg·kg-1·d-1. The survival time, pathological changes, biochemical markers and CTL, T lymphocyte apoptosis were observed. Results The postoperative survival time of paclitaxel group were rats higher than acute rejection group, (18.2±1.5)d vs(8.8± 1.9) d, P<0.01. HE score (Banff) of acute rejection group is higher than the paclitaxel group, 7.80±0.83 vs 4.80±0.82, t=5.67, P<0.01. Albumin level in paclitaxel group ranked higher than acute rejection group, P=0.003, while alanine aminotransferase of paclitaxel group were lower than acute rejection group, P<0.01. In CTL, the percentage of anti-target cell in acute rejection group were higher than the percentage of paclitaxel group, P<0.01. Tlymphocyte apoptosis rate of acute rejection group isless than the paclitaxel group, (7.16±1.56)% vs(16.47±2.51)%, P<0.01. Conclusion Paclitaxel exerts immunosuppression in rat liver transplantation, by inducing T lymphocyte apoptosis to suppress T lymphocyte activation probably. Key words: Paclitaxol; Rat; Liver transplantation; CTL