Effect of Paclitaxel along with Di allyl sulfide on Nucleic acid and polyamines changes in 7, 12 Di methyl benz(a) anthracene induced skin cancer wistar rats

Abstract

Aim: The purpose of this study is to investigate the changes in the levels of nucleic acid and polyamines and efficacy of combination of paclitaxel along with Di allyl sulfide against skin cancer in experimental animals. Skin cancer is the most common form of human cancer. It is estimated that over 1 million new cases occur annually. The annual rates of all forms of skin cancer are increasing each year, representing a growing public concern. The most common warning sign of skin cancer is a change in the appearance of the skin, such as a new growth or a sore that will not heal. Skin cancer is caused by chemical carcinogens and Papilloma virus infection. Design/Methods: Skin cancer was induced in rats by 7, 12 Di methyl benz(a) anthracene (DMBA) at the dosage of 5 µg dissolved in 100µl acetone and administered into experimental animals for 28 weeks. In this study, we demonstrated that combination of paclitaxel and Di allyl sulfide revert the changes in the rats from lethal dose of DMBA within 30 days. Results: The levels of Nucleic acid and polyamines in skin and liver were found to be increased in the cancer bearing animals when compared with control animals. Treatment of Paclitaxel along with Di allyl sulfide to cancer induced animals showed significant decrease in the levels of Nucleic acid when compared with cancer induced animals. Conclusions: The treatment with combination of paclitaxel and Di allyl sulfide effectively reduced nucleic acid and polyamines levels. So, from the obtained results it is concluded that paclitaxel and Di allyl sulfide is capable of restoring the skin architecture.