Abstract
In the freshly prepared rat renal cortical tubule suspension, the effects of ATP on intracellular free calcium mobilization and gluconeogenesis were investigated. ATP increased intracellular free calcium concentration ([Ca 2+] i) in a dose-dependent manner (10 −6 - 10 −3 M). The rank order in the potency of ATP analogs at 10 −4 M was 2-methylthio ATP>ATP≥ADP. AMP, adenosine and α,β-methylene ATP did not respond to [Ca 2+] i. These results suggest that P 2-purinoceptor in the rat kidney cortex should be specifically P 2Y subtype. Renal gluconeogenesis from pyruvate was stimulated maximally by 10 −4 M ATP(48.3 ± 13.2%). This effect was significantly inhibited by 10 −4 M suramin. Thus, the present study suggests that renal gluconeogenesis is increased via P 2Y-specific purinoceptor stimulation.
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