Abstract
In this study, combined effect of ozonolysis (Oz) and temperature on rice straw (RS) hydrolysis was studied. At ozone dosage of 0.006 gO3/g RS, 19% disintegration in the RS fiber crystallinity was observed with 17% reduction in the total carbon content. Thermal (55 °C, 4d) treatment followed after Oz showed the generation of total volatile fatty acids (VFAs) and reducing sugars of 537.20 ± 17.09 mg/L/d and 1.18 ± 0.06 mg/L/d, respectively. Biomethane assays after pretreatment of RS showed 26% enhancement in the RS hydrolysis rate constant (k = 0.1885 d−1) with methane yield of 374 ± 6 ml CH4/g-VSsubstrate. The potential scale up feasibility was estimated by calculating net energy balance. A net positive gain of 0.5763 kWh/kg RS was obtained using this pretreatment.
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