Abstract
The effects of oxygen tension and dilution rate on expression and stability of killer toxin chimeric plasmid (pADH-10A) in transformants of wine yeast (Montrachet 522) were investigated using a glucose-limited chemostat culture. Killer toxin activity increased as a function of dilution rate when transformants were grown in N2 or air, but not in 100% O2. Killer transformants grown in the presence of N2 had 2–4 fold and 10–15 fold higher toxin expression than that in the presence of air and 100% O2, respectively. Plasmid stability increased as a function of dilution rate, regardless of the oxygen tension used. However, transformants grown in air gave the highest plasmid stability.
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