Abstract
BackgroundBone damage is a condition that affects the quality of life of patients. Mesenchymal stem cells (MSCs) are important for bone repair. Osteoking is a natural compound in traditional Chinese Medicine used to treat bone diseases; however, the effect of Osteoking on the differentiation of MSCs has not been reported. In this study, we aimed to investigate the effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells (rbMSCs).MethodsThe effects of Osteoking on the proliferation and differentiation of rbMSCs were investigated. Different concentrations of Osteoking were prepared, and its cytotoxicity was evaluated by CCK-8 assay. The expression of osteogenic and adipogenic genes were determined, and several staining methods were used to reveal the osteogenic and adipogenic differentiation potential of rbMSCs.ResultsOur results show that appropriate concentrations of Osteoking can enhance osteogenic differentiation of rbMSCs and reduce adipogenic differentiation without any effect on proliferation. This may be related to the changes in related gene expression.ConclusionOsteoking enhances osteogenic differentiation and inhibits adipogenic differentiation of rbMSCs. Therefore, Osteoking may have a therapeutic potential for treating bone disease caused by changes in differentiation function of MSCs.
Highlights
Bone damage is a condition that affects the quality of life of patients
We investigated the effect of different concentrations of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow Mesenchymal stem cell (MSC) in vitro in this study
The results showed that Rat bone marrow mesenchymal stem cell (rbMSC) could differentiate into osteoblasts, adipocytes and chondrocytes
Summary
Mesenchymal stem cells (MSCs) are important for bone repair. Osteoking is a natural compound in traditional Chinese Medicine used to treat bone diseases; the effect of Osteoking on the differentiation of MSCs has not been reported. We aimed to investigate the effect of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow mesenchymal stem cells (rbMSCs). Osteoking was approved by the Chinese State Food and Drug Administration in 2002 and exhibits curative effects in the clinical treatment of different kinds of bone diseases, especially femoral head necrosis, prolapse of the lumbar intervertebral disc, fracture, and osteoarthritis [11]. MSCs, which could differentiate into osteoblasts, are one of the most important cells related to bone repair. We investigated the effect of different concentrations of Osteoking on the osteogenic and adipogenic differentiation potential of rat bone marrow MSCs (rbMSCs) in vitro in this study
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