Abstract
The diameters of 48-h-old Geotrichum candidum colonies on agar decreased markedly with decreasing water potential in the range of −1.0 to −2.5 MPa, but there was a slight increase from −0.5 to −1.0 MPa. Differences between the effects of KC1, polyethylene glycol (PEG) 400, or mannitol were small. This pattern was similar when G. candidum was grown on semipermeable membranes on agar, where the water potential is mainly osmotic, and when the fungus was grown on semipermeable membranes floating on media amended with PEG 20000, where the water potential is mainly matric. Dry weight accumulation by G. candidum on membranes on agar or on floating membranes at 48 h showed the same pattern as did colony surface area: a slight stimulation at −1.0 MPa and a decrease with further decreases in water potential. This differed from previous results with G. candidum in liquid shake culture, where no effect on dry weight accumulation between −0.5 and −2.5 MPa was observed in media amended with PEG 400 or mannitol.
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