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Abstract
Fertilization of mouse and hamster eggs in vitro in media of various calculated osmolalities was investigated. A modified Krebs\x=req- Ringer bicarbonate solution with bovine serum albumin was used for the mouse gametes and a modified Tyrode's solution with heated hamster blood serum was used for the hamster gametes. Penetration of mouse eggs could occur in media, the osmolality of which varied from 256 to 418 mosmolal, but the optimal osmolality was between 308 and 372 mosmolal. Penetration of hamster eggs could occur in media with osmolality varying from 232 to 452 mosmolal but the optimal osmolality was from 292 to 392 mosmolal. It seems that fertilization in vitro could be achieved better in hypertonic than hypotonic solutions and that hamster gametes could withstand the variation of osmolality slightly better than mouse gametes.
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