Effect of organic and inorganic farming conditions on seed storage proteins in soybean based cropping system

Abstract

A field experiment was conducted during rainy (kharif) and winter (rabi) seasons of 2011-13 to evaluate the effect of application of farmyard manure (FYM), recommended doses of fertilizers (RDF) and their combination on storage protein quality of soybean [Glycine max (L.) Merr.], chickpea (Cicer arietinum L.) and wheat (Triticum aestivum L.) in soybean based cropping system in Punjab. Densitometric analysis of chromatograms revealed significant variations in relative densities of ?' subunit of ?-conglycinin protein in soybean in year 2011 and ? subunit of legumins in chickpea among different treatments whereas other subunits of glycinin and ?-conglycinin in soybean and legumins and vicillins in chickpea did not vary significantly. Maximum value of 11S/7S ratio was observed in treatment with FYM @ 10t ha-1 and FYM @ 10t ha-1+100% RDF in soybean seeds in year 2011 and 2012 respectively and with RDF in chickpea. In wheat, the relative quantity of 55.7, 48.8 and between 11-13 kDa bands increased significantly in combined treatment of organic or inorganic fertilizer than their individual treatments, whereas relative quantity of 40.7 kDa band was significantly low in FYM @10t ha-1 +100% RDF treatment as compared to other treatments. Combined treatments of organic and inorganic fertilizer resulted in presence of additional bands corresponding to 61.4 and 22.5 kDa and disappearance of subunit band corresponding to 16.4 kDa in wheat grains. It is concluded that combined organic and inorganic treatments did not change major storage protein fractions and can be used to improve fertilizer management practices.