Effect of Organic Additives on the Micropropagation of Asparagus officinalis

Abstract

In vitro propagation is one of the most promising techniques for the large-scale clonal propagation of Asparagus officinalis. The aim of this study was to investigate the effect of organic additives, including coconut water (CW), banana homogenate (BH), and potato homogenate (PH), on A. officinalis shoot and root proliferation. The results revealed that CW, specifically at 20% (v/v), was the most effective organic additive for promoting shoot and root formation in the in vitro plantlets. Furthermore, the longest shoot and root lengths were also observed in the MS medium supplemented with 20% (v/v) CW. In the supporting medium of 1:1 peat moss and vermiculite, the in vitro plantlets exhibited a high survival rate with a morphology comparable to that of the mother plant. The results of this study demonstrate that CW can be applied as a supplemental material for large-scale A. officinalis micropropagation.