Effect of Orento, a Traditional Japanese Medicine, on IL-6, IL-8 Secretion, Type 1 Collagen Production and Alkaline Phosphatase Secretion in the Human Osteosarcoma Cell Line Saos-2.

Hourei Oh,Nobutaka Okusa,Kazuya Masuno,Yoshimasa Makita,Yasuhiro Imamura,Shin-Ichi Fujiwara

doi:10.3390/medicines7100061

Hourei Oh, Nobutaka Okusa + Show 4 more

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https://doi.org/10.3390/medicines7100061

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Journal: Medicines	Publication Date: Sep 25, 2020
Citations: 2	License type: CC BY 4.0

Affiliation: Osaka Dental University, Matsumoto Dental University

Abstract

Background: Orento, a traditional Japanese medicine, is known as Kampo medicine in Japan. We investigated the possible efficacy of Kampo medicine for periodontal disease. In this study, we examined the in vitro effects of orento on the proliferation of the inflammatory cytokines interleukin (IL)-6 and IL-8, the production of type 1 collagen, and the secretion of alkaline phosphatase (ALP) in the human osteosarcoma cell line Saos-2 (Saos-2 cells). Methods: The proliferation of Saos-2 cells was assessed by MTT assay. IL-6 and IL-8 levels, type 1 collagen production and ALP secretion were evaluated using enzyme-linked immunosorbent assay and ALP assays. Saos-2 cells were treated with or without 0.1, 1, 10, 100 and 1000 μg/mL of orento for 24 h. Results: Orento (10 μg/mL) significantly induced the proliferation of Saos-2 cells. At this concentration, orento suppressed IL-6 and IL-8 and enhanced type 1 collagen production and ALP secretion. Conclusions: These results indicate that orento controls the IL-6 and IL-8 secretion and cellular metabolism of osteoblasts, resulting in the secretion of early bone-related biomarkers.

Highlights

Kampo medicines, or traditional Japanese medicine, originated from ancient Chinese medicine.Kampo medicines have characteristics such as abdominal diagnosis for therapeutic indications and useful formulas with smaller amounts of herbs, compared with Chinese medicine [1,2,3]
We previously established a human gingival fibroblast culture system stimulated with the lipopolysaccharide (LPS), an endotoxin, of the periodontitis-associated bacteria Porphyromonas gingivalis [6]
Using this system as an in vitro cellular model for periodontal disease, we demonstrated the efficacy of a variety of herbal medicines, including shosaikoto, orengedokuto, hangeshashinto, hochuekkito, hainosankyuto and orento, for the treatment of periodontitis [7]

Summary

Introduction

Traditional Japanese medicine, originated from ancient Chinese medicine. Orento is a type of Kampo medicine composed of seven crude drugs. These include Coptis rhizome, processed ginger, cinnamon bark, Pinellia tuber, ginseng, Glycyrrhiza and jujube. We previously established a human gingival fibroblast culture system stimulated with the lipopolysaccharide (LPS), an endotoxin, of the periodontitis-associated bacteria Porphyromonas gingivalis [6]. Using this system as an in vitro cellular model for periodontal disease, we demonstrated the efficacy of a variety of herbal medicines, including shosaikoto, orengedokuto, hangeshashinto, hochuekkito, hainosankyuto and orento, for the treatment of periodontitis [7]. We investigated the in vitro effect of orento on IL-6 and IL-8 secretion, type 1 collagen production and ALP secretion in Saos-2 cells

Cell Cultures

Reagents

MTT Assay

Statistical

Results

4.4.Discussion