Effect of Orally Dosage from Anise Fruit Extract and Alginate Nanoparticles on Some Biological Parameters of Hamster Males

Abstract

The study was conducted to determined the effect of oral dosage of alcoholic anise fruit extract or Alginate nanoparticles (AlgNPs) and their interaction with the antibiotics of Cefotaxime or Metronidazole on the biological parameters of male Hamster Males experimentally infected induced with diarrhea caused by E. coli. The results showed that there was a significant decrease (p<0.05) in hemoglobin, red blood cell count and PCV in the blood of a group of Hamster Males that induced diarrhea, it was at 10.75 g/dL, 4.14 cells/mm3 and 37%, respectively compared with their values in the group of control animals, which were 14.25 g/dL and 6.72 cells/mm3 46%, respectively. Oral dosage to infected animals of anise fruit extract and AlgNPs led to a significant increase in hemoglobin, red blood cell count and PCV to normal level. Also, it was found that orally dosage of laboratory animals with E.coli bacterium were caused a significant increase in the WBCs, liver enzymes activity ALT and AST, and the level of urea, as they were at 12.6 × 103 cells/mm3, 112 and 143 (IU/L) and 74.5 mg/100ml, respectively, in the group of animals infected with E.coli, compared with its same values in the control group, which were appeared at 8.6×103 cells/mm3, 44.5, 48.5 (IU/L) and 39.5 mg/100 mL, respectively.Dosing with alcoholic extract of anise fruit and AlgNPs alone or with both antibiotics Cefotaxime or Metronidazole caused a significant decrease in WBCs accounts, ALT, AST, and urea levels, which became not significantly different from their same values in the control group.