Effect of oophorosalpingo-hysterectomy on serum antioxidant enzymes in female dogs

Linaloe Guadalupe Manzano Pech,Sara Del Carmen Caballero-Chacón,Verónica Guarner-Lans,Adrián Moreno Gómez,Eulises Díaz-Díaz,Israel Pérez-Torres

doi:10.1038/s41598-019-46204-w

Abstract

There are few studies evaluating the oxidant-antioxidant status after oophorosalpingohysterectomy (OSH) in female dogs. Here we determined the effect of OSH on antioxidant enzymes in serum, and quantified morphological changes in subcutaneous adipocytes. Lateral OSH was performed in 12 female dogs. The concentration of 17β-estradiol (17β-E2), the activities of extracellular superoxide dismutase (SOD-ec), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and glutathione reductase (GR) were determined. Glutathione (GSH), glutathione disulfide (GSSG), lipid peroxidation (LPO), total antioxidant capacity (TAC), carbonylation and vitamin C were measured in serum. Subcutaneous adipose tissue was obtained to determine morphological changes and cell number, under basal conditions and six months after OSH. The SOD-ec, GPx and GST activities increased significantly (p ≤ 0.05), LPO, carbonylation and GSSG also increased. GSH and vitamin C decreased (p = 0.03). 17β-E2 tended to decrease six months after OSH. Hypertrophy of subcutaneous adipocytes was observed after OSH from the first month and was accentuated after six months (p = 0.001). The results suggest that 17β-E2 decreases after OSH and alters the antioxidant enzyme activities in serum thus, redox balance is altered. These changes are associated with an increase in body weight and hypertrophy of subcutaneous adipose tissue.

Highlights

Oxidative stress (OS) is the consequence of an imbalance between the production of reactive oxygen species (ROS) and the total antioxidant capacity (TAC) of the organism
Insulin and the HOMA index were increased in the first month after OSH with a significant difference (p = 0.01). 17β-E2 showed no significant difference, a tendency to decrease was observed in the first and sixth months after OSH
The cytologies show basal cells characteristic of the anestrus stage; this stage is the time between diestrus and the proestrus and is the recommended time for the performance of the OSH

Summary

Introduction

Oxidative stress (OS) is the consequence of an imbalance between the production of reactive oxygen species (ROS) and the total antioxidant capacity (TAC) of the organism. The goal of this study was to evaluate the changes in some serum antioxidant enzymes one and six months after surgery and in the morphology of subcutaneous adipocytes before and six months after OSH in young female dogs.

Objectives

Results

Conclusion