Abstract
The work aimed to study the effect of oocytes quality and the presence or removal of cumulus cells on the meiotic competence of vitrified-thawed immature buffalo oocytes. In experiment 1, Immature buffalo oocytes were classified according to their quality ( group 1: excellent and good, group2: fair & denuded) the groups were vitrified using 20% Ethylen Glycol (EG) and 20% Dimethyle sulphoxide (DEMSO), then thawing after one week and in vitro matured in ISCOVE medium and kept at 38.5°C under 5% CO2 for 22 h. The two groups were compared with the control without vitrification (in-vitro matured oocytes). Experiment 2, excellent and good immature buffalo oocytes were divided into two groups, G1: oocytes with cumulus, G2: oocytes without cumulus then vitrified / thawed as experiment 1, for in vitro maturation assessment. In experiment 1, The excellent and good quality immature oocytes showed significant (p < 0.05) higher in-vitro maturation rate after thawing than fair and denuded buffalo oocytes also vitrification led to loss of oocytes and abnormalities of buffalo oocytes compared to the control. In experiment 2, the presence of cumulus cells was significantly higher in (P<0.05) survivability and maturation rate than the oocytes without cumulus cells. In conclusion, selection of excellent and good quality oocytes with cumulus cells improve post thawing development of vitrified immature buffalo oocytes.
Highlights
Worldwide, there is a growing awareness of the importance of the buffalo (Bubalus bubalis), which participates in Egyptian economy, contributing milk, meat and draught power for the agriculture sector (Zoheir et al, 2007)
The work aimed to study the effect of oocytes quality and the presence or removal of cumulus cells on the meiotic competence of vitrified-thawed immature buffalo oocytes
In experiment 1, Immature buffalo oocytes were classified according to their quality the groups were vitrified using 20% Ethylen Glycol (EG) and 20% Dimethyle sulphoxide (DEMSO), thawing after one week and in vitro matured in ISCOVE medium and kept at 38.5°C under 5% CO2 for 22 h
Summary
There is a growing awareness of the importance of the buffalo (Bubalus bubalis), which participates in Egyptian economy, contributing milk, meat and draught power for the agriculture sector (Zoheir et al, 2007). Cryopreservation of oocytes collected from the ovaries of such animals and their subsequent utilization for production of embryos and for transfer may provide an opportunity to replenish the valuable germplasm lost and the genetic improvement of the species (Wani et al, 2004). Oocyte cryopreservation has some problems such as the formation of ice crystal, osmotic shock and ultra-structural damage, all of those lead to low survival and/or poor oocytes meiotic competence (Szurek et al, 2011). The ability to identify good quality oocytes is important to undergo normal in-vitro maturation; fertilization and development to the blastocyst stage (Sianturi et al, 2002). The aims of this work are to study 1) Effect of vitrification and oocytes quality on meiotic competence of vitrified-thawed immature buffalo oocytes. 2) Effect of presence or absence of cumulus cells on meiotic competence of vitrified immature buffalo oocytes The aims of this work are to study 1) Effect of vitrification and oocytes quality on meiotic competence of vitrified-thawed immature buffalo oocytes. 2) Effect of presence or absence of cumulus cells on meiotic competence of vitrified immature buffalo oocytes
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