Abstract
The work aimed to study the effect of oocytes quality and the presence or removal of cumulus cells on the meiotic competence of vitrified-thawed immature buffalo oocytes. In experiment 1, Immature buffalo oocytes were classified according to their quality ( group 1: excellent and good, group2: fair & denuded) the groups were vitrified using 20% Ethylen Glycol (EG) and 20% Dimethyle sulphoxide (DEMSO), then thawing after one week and in vitro matured in ISCOVE medium and kept at 38.5°C under 5% CO2 for 22 h. The two groups were compared with the control without vitrification (in-vitro matured oocytes). Experiment 2, excellent and good immature buffalo oocytes were divided into two groups, G1: oocytes with cumulus, G2: oocytes without cumulus then vitrified / thawed as experiment 1, for in vitro maturation assessment. In experiment 1, The excellent and good quality immature oocytes showed significant (p < 0.05) higher in-vitro maturation rate after thawing than fair and denuded buffalo oocytes also vitrification led to loss of oocytes and abnormalities of buffalo oocytes compared to the control. In experiment 2, the presence of cumulus cells was significantly higher in (P<0.05) survivability and maturation rate than the oocytes without cumulus cells. In conclusion, selection of excellent and good quality oocytes with cumulus cells improve post thawing development of vitrified immature buffalo oocytes.
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