Effect of onion extract and hydrogen peroxide on &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; isolated from urinary tract infection

Mohammed Oudah Hamad

doi:10.3329/bsmmuj.v9i4.30147

Abstract

Antibacterial activity of the mixture of onion (Allium cepae) extract and hydrogen peroxide was tested on multidrug resistant isolate of P. aeruginosa, obtained from biofilm on medical devices and urinary tract infection (UTI). P. aeruginosa exhibited high resistance rate toward sefitrixone and ciprofloxacin with inhibition zone of resistant of isolates in disc diffusion method was detected at 8 and 13 mm, respectively. While inhibition zone of imipenem for the isolated bacteria was 20 mm. Their decontamination was tested using a mixture of onion leaves aqueous extract 10.7% (w/v) with 1.0% hydrogen peroxide. The mixture showed inhibition of the isolates. It can be concluded that a special antibacterial solution could eradicate multidrug resistant isolate of P. aeruginosa.

Highlights

Materials and MethodsUrinary tract infection (UTI) is a common community-acquired bacterial disease, which frequently affect female outpatient
Pseudomonas aeruginosa is the most common member of the family Pseudomonas disease accounts of the majority of urinary tract infections
Development of resistance may occur during antimicrobial therapy and is well documented during monotherapy, increasingly clear that resistance development in P. aeruginosa is multifactorial with mutations in genes encoding prions, efflux pumps, penicillin-binding proteins, and chromosomal beta–lactamase.[1]

Summary

Introduction

Materials and MethodsUrinary tract infection (UTI) is a common community-acquired bacterial disease, which frequently affect female outpatient. This study was designed to find out the antibacterial activity of onion and hydrogen peroxide combination on the multidrug resistant P. aeruginosa bacteria associated with urinary tract infection. MullerHinton agar well was filled with 0.1 mL of prepared concentrations for each mixture (onion extract 32.5 g/L and 1.0% hydrogen peroxide) and incubated the plates at 37°C for 24 hours.

Results

Conclusion