Effect of O-linked N-acetylglucosamine pathway in the rats acute renal injury induced by cecal ligation and puncture

Abstract

Objective To observe the protection effect of increasing activity of O-linked N-acetylglucosamine (O-GlcNAc) pathway by glutamine treatment in the rats acute renal injury induced by cecal ligation and puncture (CLP).Methods Seventy six healthy Sprague-Dawley rats were randomly divided into five groups:the CLP group (group C,n=16) receiving CLP,Shamoperation group (group S,n=12),the glutamine group (group G,n=16) receiving CLP and intravenous injection of 0.75 g/kg glutamine in 1 h,the quercetin and glutamine treatment group (group Q,n=16) receiving peritoneal injection of 0.4 g/kg quercetin before glutamine treatment,the alloxan and glutamine treatment group (group A,n=16) receiving peritoneal injection of 90 mg/kg alloxan before glutamine treatment.All rats were used general anaesthesia and MAP were monitored for 16 h.After 24 h,the survival rats were anesthetized again to draw arterial for gas analysis and Cr,and urine for kidney injury molecule-1 (KIM-1) were detected.The kidney was reserved to detect the expression of O-GlcNAc and HSP70 by Western Blotting after the rats were sacrificed.Results In group S,the MAP was stable,and the lactic acid at 24 h was nomal.In group C,G,Q and A,the MAP were descent obvious at 16 h after CLP,and the lactic acid of those groups increased at the 24th timepoint compared to group S (P＜0.05).Compared with group S,the Cr(57.4±4.9) mmol/L and KIM-1(66.3±8.8) ng/L increased in the group C(P＜0.05).In group G,the Cr and KIM-1 decressed but the expression of O-GlcNAc and HSP70 were increased compared with group C (P＜0.05).In group Q and group A,the Cr[(51.4±2.9),(57.4±2.6) mmol/L] and KIM-1[(70.9±17.7),(75.3±10.9) ng/L] increased Compared with group G (P＜0.05).The expression of O-GlcNAc in the group A,and HSP in the two groups decreased compared with group G (P＜0.05).Conclusions Glutamine may induces the expression of HSP70 in rats by increasing the activities of O-GlcNAc pathway,then to ameliorate Inflammation,protest the kidney. Key words: Sepsis; Acute kidney injury; Glutamine; O-linked N-acetylglucosamine pathway