Effect of Oligosaccharide Modified Material X on Viability of Human Cancer Cell Lines

Abstract

Anticarcinoma agents take part in the selective destruction of cancer cell lines, or inhibit the growth and proliferation of cancer cells. Finding anticarcinoma agents that have do not have noteworthy negative side effects is important matter for application in various flelds. Most of polysaccharides were used as medical product or an additive to health functional food. For example chitin and chitosan are known to exhibit antitumor, antibacterial, and antihypertensive activity. In this study, we incubated two kinds of cancer cells (Hep3B, A549) and 293T HEK cell treated with material X in concentration of 0.5%, 1% and 2% respectively for 24h. Then we measured viability of the cells by MTT assay. Our data suggest viability of 293T decreases gradually as concentration of X increases. Survival rates of 293T with X in concentration of 0.5%, 1% and 2% are 73.3%, 53.2% and 31.3% respectively. The cancer cell lines had more tolerance for 0.5% X and 1% X. However the cancer cells exhibited a rapid decline of viability when treated with 2% X. Survival rate of Hep3B with 2% X is 8.7% and that of A549 is 8.4%. With 2% X, cancer cell lines are about 4 times cytotoxic effects of the normal cell line. These results indicate that material X of specific concentration or higher depletes cancer cell lines while showing gradual effect on normal cell lines. To our knowledge, material X could be a promising antitumor application.