Effect of oestradiol treatment during GnRH-induced ovulation on subsequent PGF 2 α release and luteal life span in anoestrous ewes

Abstract

In sheep, induction of ovulation during anoestrus is accompanied by a high incidence of short luteal phases, though pre-treatment with progesterone can overcome this problem. We have investigated the effects of supplementing oestradiol during GnRH-induced ovulation on subsequent PGF 2 α release and luteal life span. Thirty anoestrous crossbred ewes received 250 ng GnRH i.v. at 2 h intervals for 48 h to induce ovulation either alone (group 1; n=10) or in association with either an i.m. injection of 20 mg progesterone 3 days earlier (group 2; n=10) or 3 i.m. injections of 10 μg oestradiol at 8 h intervals on the second day of GnRH treatment (group 3; n=10). Laparoscopy, performed 3 days following GnRH to confirm ovulation and 8 days later, coupled with plasma progesterone analysis were used to determine luteal life span. On day 4 following GnRH, plasma samples were collected at 20 min intervals for 8 h to monitor PGF 2 α release. One ewe from group 1 failed to ovulate and was excluded from further analysis. All groups showed an increase ( P<0.01) in plasma oestradiol during GnRH treatment, with group 3 showing a marked ( P<0.001) increase over that seen in the other two groups. In group 1 there were 1.4±0.2 PGF 2 α episodes/ewe/8 h. In group 2, pre-treatment with progesterone caused the complete inhibition of PGF 2 α episodes (0 episodes/ewe/8 h) while in group 3, treatment with oestradiol resulted in a significant reduction (0.3±0.1 episodes/ewe/8 h) compared with group 1 ( P<0.01). In group 1, 9/9 ewes exhibited short cycles compared with 2/10 ewes in group 2 ( P<0.01). In group 3 the proportion of ewes showing short cycles 7/10 ewes was not significantly different from the other groups. While treatment with oestradiol caused a significant attenuation of PGF 2 α release, this was associated with only a partial reduction in the incidence of short cycles.