Effect of Nutrients on Biomass Production of Helminthosporium Tetramera A Leaf Spot Pathogen of Sugarcane (Saccharum Officinarum)

Dr Abhijeet Bajeerao Pawar

doi:10.47191/ijcsrr/v4-i5-09

Abstract

Abstract : Helminthosporium tetramera was a pathogen of Saccharum Officinarum, was isolated from diseased Saccharum Officinarum, leaves from Nashik district and used for the present study. Pathogen was grown on the Czapek-Dox liquid medium substituting or adding different carbon, nitrogen to study biomass production. The growth as dry mycelial biomass was observed on the 8th day of incubation period. A grate extent of growth variation was observed on different carbon, nitrogen. Among the carbon source, fructose shows maximum biomass while glucose with minimum biomass. From nitrogen source cobalt nitrate and barium nitrate shows maximum and control condition with minimum biomass was recorded.

Highlights

Saccharum officinarum is a large, strong-growing species of grass in the genus Saccharum
Helminthosporium tetramera was grown on Czapek-Dox liquid medium and dry biomass was observed for 10 days (Table-1) more rapid growth was observed during early stages and maximum growth was observed on eight day
Data in the table - 2 indicates that there was large variation in the growth of Helminthosporium tetramera on different carbon compounds. This pathogen shows maximum growth on fructose followed by lactose, dextrose, glucose and control while in absence of carbon source there was no growth

Summary

Introduction

Saccharum officinarum is a large, strong-growing species of grass in the genus Saccharum. Its stout stalks are rich in sucrose. This plant affected by many fungi as leaf spot. Pathogen Helminthosporium tetramera was responsible for the brown spot on the sugarcane leaves. The fungus grows both intra and inters cellular within the tissues. Conidiophores arise as lateral branches from the hyphae and emerge through stomata or wounds. Conidiophores are short, erect, branched only at bases, short, segmented, dark brown to olivaceous at the base and somewhat paler at the growing tip. Slightly curved; tapering towards the rounded ends and vary greatly both in size as well as in the number of septa. Many worker reviewed physiology and biochemistry of fungi (Stall,1958; Rajderkar,1966; sharma et al.,1985; Sankaran et al.,1986; Nair and Sumaridi, 2000; Bhanumathi, 2007; Mantri, 1969; Jayraj and Ramabadran, 1998)

Methods

Results

Conclusion