Effect of Norfloxacin on Carbon-source Metabolic Functional Diversity of Soil Microbial Communities

Abstract

The experiment was aimed to investigate the effects of norfloxacin(NOR) on microbial community diversity in soil.The carbon-source metabolic ability and functional diversity of soil microbial communities were analyzed by BIOLOG method.BIOLOG analysis revealed that the average well color development(AWCD) of groups treated with NOR were significantly(P0.05) lower than those in control(CK) after 7,21 and 35 days.The results showed that the richness indexes and diversity indexes of groups treated with NOR concentrations of 1 μg g-1,10 μg g-1 and 100 μg g-1 were significantly(P0.05) lower than those in CK after 7 days,and groups treated with NOR concentrations of 10 μg g-1 and 100 μg g-1 were significantly lower than those in CK after 21 days,group treated with NOR concentrations of 100 μg g-1 was significantly lower than those in CK after 35 days,respectively.It demonstrated that the carbon-source metabolic capacity and functional diversity of soil microbial communities were weaken after soils amended with NOR.However,the effects were different in groups amended with NOR.It was concluded that a certain accumulation of NOR in soil,for instance,100 μg g-1NOR performed irreversible and long-term effects on the metabolic functions of the soil microbial communities.