Abstract
We evaluated the contribution of nonpathogenic Escherichia coli O83 after coloniza- tion of germ-free (GF) rat pups and piglets on development of terminal α2,6- and α2,3-sialylated and broad range of terminal α1,2-, core α1,6-, and α1,3-, α1,4-fucosylated glycoconjugates in the suckling period relative to noncolonized GF and conventional (CV) counterparts. The ELISA-lectin approach was used to specify and quantify sialylated and fucosylated glycans in brush-border membrane vesicles (BBMV). Levels of pro- and anti-inflammatory cytokines in sera and splenocyte secretions were demonstrated using ELISA. Rat and pig intestinal responses to E. coli O83 monoassociation were different from those of GF and more similar to the CV animals in the decline of sialylated glycans, which is in agreement with the shorter life span of enterocytes in E. coli O83 monoassociated rats and pigs. No significant effect of E. coli O83 colonization on labeling fucosylated glycans was observed for immature fucosylation at the late suckling period. We demonstrate spontaneous secretion of pro-inflammatory cytokine IL-18 and anti-inflammatory IL-10 by GF rat splenocytes, and its suppression of IL-18 in E. coli O83 associated rat pups, and suggest that these cytokines serve as an immunomodulatory pool during the suckling period indicating the balance between T helper (Th) 1 and Th2 phenotypes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: International Journal of Interferon, Cytokine and Mediator Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.