Effect of non surgical periodontal therapy on salivary and RBC lysate superoxide dismutase levels in periodontitis: A clinico biochemical study

Abstract

To compute superoxide dismutase (SOD) levels in saliva and plasma of chronic periodontitis patients as compared to healthy controls, to find the association, if any, with clinical parameters and to verify the role of non surgical periodontal therapy (NSPT) in amending SOD activity. Forty two subjects were divided into two groups based on periodontal parameters; Group A: 20 chronic periodontitis patients (CP) and Group B: 22 periodontally healthy controls (PH).Clinical parameters, saliva samples (modified draining method) and plasma samples (by venipuncture) were evaluated at baseline, at 3 months and 6 months after NSPT for changes in clinical parameters and SOD activity. SOD levels were determined by Mc Cord and Fridovich method and absorbance was read by spectrophotometer at 560 nm. Significantly lower levels of salivary and plasma SOD (p<0.05) were detected in CP patients with a negative co-relation with clinical parameters at baseline in group-A. Post therapy SOD levels increased significantly (p<0.05) and plasma SOD levels rose even above that of control group.