Effect of nitrogen source on short-term light and dark CO2 uptake by a marine diatom

Abstract

Based on a series of short-term incubations involving the marine diatom Chaetoceros simplex (Bbsm), precultured in NH 4 + -, NO 3 - -and urea-limited continuous cultures at several dilution rates, we found that both the short-term specific rate of 14CO2 uptake and the amount of CO2 fixed after 8- and 16-min incubations were unaffected by enrichment with NH 4 + , urea, or NO 3 - when NH 4 + or urea were the preconditioning forms of N, but were slightly suppressed when the cells were first grown on NO 3 - . Similar enrichments in the dark, however, led to significant CO2 uptake under all conditions of NH 4 + enrichment and to similarly enhanced CO2 uptake, but only at high growth rates, when urea was the source of enrichment nitrogen. Our light results are contrary to some contemporary findings, but there does seem to be agreement that photosynthetic rates of rapidly growing phytoplankton will not be affected by exposure to pulses of nitrogen. Enhanced dark uptake, in contrast, appears to be characteristic of phytoplankton under all degrees of N limitation, and, as such, may be useful as an “all or nothing” index of the nitrogen status of natural waters. There is some indication that the index may be useful in determining both the form of and the degree of N limitation as well.