Abstract
The effect in vitro of nitrogen mustard (HN2) treatment on physical parameters of partially purified hyaluronate from human umbilical cords and of synovial fluid from patients with rheumatoid arthritis was studied. Intrinsic viscosities, gel filtration elution patterns (Sepharose 2B) and acrylamide gel disk electrophoresis runs on HN2-treated (1 mg/ml HN2, 0.9% saline, 8 hr at 37°) and control hyaluronate were obtained. The intrinsic viscosity was reduced by 10 to 50 per per and a typical gel filtration elution pattern showed retardation by about 10 per cent after HN2 treatment. No gross difference in disk electrophoretic pattern was noted. Similar intrinsic viscosity changes were observed after HN2 treatment of rheumatoid synovial fluids.
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