Abstract

Rumen microbes utilize mainly ammonia as a nitrogen (N) source for their growth but some species also use a variety of amino acids (AA) or peptides. Several studies have shown both an enhanced fibre digestion and efficiency of rumen micro-organisms when N sources such as AA, peptides and protein were provided in addition to ammonia (Griswold et al.,1996). However, no difference either in digestion or in growth of rumen microbes was found in other in vivo(Fujimaki et al.,1989) and in vitro (Kernick, 1991) studies. The objective of this experiment was to investigate the effects of protein, peptides, AA and ammonia on microbial growth and fermentation of an all fibre basal diet in a semicontinuous culture system (RUSITEC; Czerkawski and Breckenridge, 1977).

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