Effect of Nitrobenzene granules and Seaweed extracts on biochemical contents of Arachis hypogaea callus culture.

Abstract

The present study is aimed to evaluate the effect of organic extracts (benzene, diethyl ether and water) of seaweeds (Halimeda gracilis, Ceramium rubrum and Cystophyllum muricatum) and nitrobenzene granuleson biochemical contents of Arachishypogea L. callusunder in vitro conditions. The callus of Arachishypogea L. was obtained from the leaf explants on MS medium containing 2, 4-D (1 mgL-1) and BAP (0.5 mg L-1). The mass multiplication of callus was achieved at 1mg L-1 of 2, 4-D and 0.5 mg L-1 of GA3. The calli were then treated with different concentrations (0.5, 1.0 and 1.5 mg L-1) of seaweed extracts and Nitrobenzene granules. Total carbohydrate, total protein and total chlorophyll contents were analyzed at 5, 10 and 15 days intervals. The total carbohydrate content was high (3.7mg/100mg) in callus treated with Benzene extract of Ceramium rubrumat 1.5 mg L-1 on 15th day. The total protein content was increased (6.9mg/100 mg) in callus treated with Benzene extract of Cystophyllum muricatum at 0.5 mg L-1 on 5th day and the total chlorophyll content was lower (0.36mg/100mg) in Nitrobenzene granules at 0.5mg L-1 in 5th day when compare to control. The present study reveals the positive role of different extracts of seaweeds on increasing the biochemical contents of callus culture of A.hypogea. The extracts can be further evaluated for their role on enhanced regeneration of plants from callus culture.