Abstract
1. 1. The effect of nitrite on the mitochondrial oxidation of succinate and of ascorbate plus tetramethyl- p-phenylenediamine (TMPD) was investigated using tightly-coupled ox neck muscle mitochondria. 2. 2. Both respiration and oxidative phosphorylation were inhibited by nitrite, the effect being more pronounced at a low pH. 3. 3. The oxidation of ascorbate plus TMPD was more sensitive to nitrite than the oxidation of succinate at both pH 7.2 and at pH 6.1. 4. 4. At pH 6.1, the State 3 respiration for the oxidation of ascorbate plus TMPD was completely inhibited by 3.75 μmoles nitrite per mg. protein as compared with 15.50 μmoles nitrite per mg. protein for the oxidation of succinate.
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