Abstract
Nitric oxide (NO) is a gaseous signaling molecule in plants that plays a key role in mediating a wide range of physiological processes and responses to biotic and abiotic stresses. The present study was conducted to investigate the effects of the exogenous application of sodium nitroprusside (SNP), an NO donor, on cadmium (Cd)-induced oxidative stress and Cd uptake in rice plants. Rice plants were exposed to Cd stress (0.2 mmol L−1 CdCl2) and different concentrations of SNP (0.05, 0.1, 0.2, and 0.4 mmol L−1). A SNP concentration of 0.1 mmol L−1 (SNP10) significantly reduced the Cd-induced decrease in shoot and root dry weights and leaf chlorophyll concentrations. The addition of NO also reduced the malondialdehyde (MDA), hydrogen peroxide (H2O2) and ascorbic acid (ASA) concentrations. However, the reduction in glutathione (GSH) concentration was inhibited by NO treatment. Moreover, NO prevented the Cd-induced increase in antioxidative enzyme activity. The amount of Cd accumulation in rice plants was also influenced by the addition of NO. The NO supplied by the SNP enhanced the Cd tolerance of the rice by increasing the Cd uptake by the roots and decreasing the Cd accumulation by the shoots. However, the application of potassium ferrocyanide (Cd+Fe) or sodium nitrate and nitrite (Cd+N) (without NO release), did not exhibit the effects of the SNP. Furthermore, the effects of the SNP were reversed by the addition of hemoglobin (an NO scavenger). Our results suggested that exogenous NO was involved in the resistance of rice to Cd-toxicity.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.