Effect of ningdong granule on 5-hydroxytryptamine metabolism and 5-HT 2AR of tourette’s syndrome rat models

Abstract

Objective To investigate the effect of Ningdong granule on 5-hydroxytryptamine metabolism and 5-HT2AR of Tourette’s Syndrome（TS） rat models. Methods 32 SD rats were divided into 4 groups randomly, the control group, the model （Apo） group, the low dosage NDG （NGL） group, and the high dosage （NGH） group. TS rat models were induced by intraperitoneal injection （i.p.） with Apo （2 mg/kg） in the experimental groups last 4 weeks. Rats in the control group were injected with normal saline （0.9%） （3 ml/kg, i.p.）. Then, the rats were treated by intragastric administration （i.g.） with ND granular at 1.8g/（kg？d）in NGL group, with ND granular at 3.6 g/（kg？d）in NGH group, with distilled water at equal Volume in Control group and Apo group respectively for 12 successive weeks. Analysis the striatum 5-HT2AR protein expression by Immunohistochemical method;Determination of the content of 5-HT and 5-HIAA in striatum by Enzyme linked immunosorbent assay （ELISA）. Results The level of 5-HT and 5-HT2AR protein expression in the model group [（1.98±0.14）ng/ml and（143.10±8.01）], compared with the control group[（1.78±0.10）ng/ml and（127.43±6.72）], was up-regulated （P〈0.01 or 0.05）. The levels of 5-HT and 5-HT2AR protein expression in NGH group[（2.20±0.14）ng/ml and（166.33±9.21）ng/ml] were further up-regulated, and the levels of＆amp;nbsp;5-HIAA in NDH group [（0.58±0.08） ng/ml] became lower, compared with the control group（P<0.01 or 0.05）. Conclusion Activity of 5-HT system enhanced in Apo-induced TS rat model. However, the enhanced activity may still be relatively insufficient. The mechanism of ND granulear treating TS was associated with depression of the 5-HT metabolism, up-regulation expression of 5-HT2AR, and improving the activity insufficient of 5-HT system. Key words: Ningdong granule; Tourette’s syndrome; 5-hydroxytryptamine; 5-HT2A receptor