Effect of Nigella sativa Supplementation on Oxidative Stress and Antioxidant Parameters: A Meta-Analysis of Randomized Controlled Trials.

M. J. Al-Farabi,M. Ardiana,H. O. Hermawan,B. S. Pikir,A. Santoso

doi:10.1155/2020/2390706

Abstract

Introduction Nigella sativa is a commonly used traditional medicine which has been shown to have antioxidant properties. However, its supplementation in patients of clinical trials showed conflicting results. Materials and Method. Relevant articles were searched through PubMed/Medline, SCOPUS, and Google Scholar databases using “Nigella sativa” or “black seed” or “black caraway” or “thymoquinone” and “oxidative stress” or “antioxidant” and “clinical trial” keywords. Randomized, placebo-controlled human interventions using Nigella sativa were included in this study. The methodological quality of studies was assessed using Jadad's quality scales. Results Five studies using 293 subjects met the inclusion criteria. The overall quality of all included trials was determined based on the low risk of bias and the high quality of reported information (Jadad score ≥ 3). Meta-analysis of 293 eligible subjects showed that treatment with Nigella sativa improved the superoxide dismutase (SOD) level (48.18; 95% CI 30.29 to 66.08; p < 0.01), but there was no significant effect on the malondialdehyde (MDA) level (−5.32; 95% CI −1.19 to 0.128; p=0.114) and total antioxidant capacity (TAC) level (0.219; 95% CI −0.136 to 0.573; p = 0.227). Conclusion This meta-analysis suggests that Nigella sativa supplementation in humans may benefit as an antioxidant by increasing SOD levels but has no significant effect on the MDA level and TAC level.

Highlights

Nigella sativa is a commonly used traditional medicine which has been shown to have antioxidant properties
Other studies failed to find significant changes in the MDA, glutathione peroxidase (GPx), and total antioxidant capacity (TAC) [13, 15,16,17]. ere is no previous meta-analysis that summarized the antioxidant capabilities of Nigella sativa (NS) supplementation. erefore, this systematic review and meta-analysis is conducted to investigate the effects of NS supplementation on oxidative stress parameters such as MDA and the production of antioxidant enzymes represented by superoxide dismutase (SOD) and total antioxidant capacity (TAC) in a randomized controlled trial (RCT)
We found 167 relevant records

Summary

Introduction

Nigella sativa (NS), which is commonly called black caraway, is a traditional medicine that has been used in Middle Eastern countries, Asia, Southern Europe, India, Pakistan, Syria, Saudi Arabia, Turkey, and the Southern Mediterranean countries. Erefore, this systematic review and meta-analysis is conducted to investigate the effects of NS supplementation on oxidative stress parameters such as MDA and the production of antioxidant enzymes represented by SOD and total antioxidant capacity (TAC) in a randomized controlled trial (RCT). Studies were selected if they met these criteria: (1) full-text publication written in English; (2) randomized controlled trials (either parallel or crossover); (3) conducted on ≥18 year-old subjects; (4) evaluate the effects of NS on oxidative parameters and/or antioxidant enzymes; (5) reported parameters before and after the intervention in both placebo and treatment groups. E following data were extracted from the included studies by using standardized protocol: first author’s name; year of publication; study location; study duration; age and gender of participants; study design; health status of study population; number of participants in each groups; type and dose of NS supplements; and mean ± standard deviation (SD) and p value of oxidative stress and antioxidant enzyme parameters. Ethical Approval. is meta-analysis did not require ethical approval since all of the data retrieved for the studies were already available in the public domain

Results

Discussion