Abstract
Background: In recent years, nickel (Ni) has been widely applied in industrial and agricultural production and have became a kind of environmental pollution. In this study, the effect of nickel chloride (NiCl 2 ) with different concentration on Arabidopsis genomic stability and DNA methylation has been demonstrated. The nucleolus variation and 18S rDNA methylation after NiCl 2 treatment have been analyzed. Results: The results are as follows: (1) The NiCl 2 could result in heritable genomic methylation variations. The genomic DNA methylation variations have been detected by methylation-sensitive amplified polymorphism (MSAP) molecular markers, and the result showed that after NiCl 2 treatment, there was methylation variation in T 0 generation seedlings, and partial sites changes maintained in T 1 generation, which suggested that the effects of NiCl 2 on DNA methylation could be heritable in offspring. (2) NiCl 2 brought deformity and damage to nucleolar structure in Arabidopsis root tip cells, and the damage was positively correlated with the NiCl 2 concentration. 3. In the nucleolus, there was increased cytosine methylation in 18S rDNA. The plant nucleolus variation and 18S rDNA methylation maybe be used as an examination indicator for Ni pollution in soil or plant. Conclusions: NiCl 2 application caused variation of DNA methylation of the Arabidopsis genomic and offspring's. NiCl 2 also resulted in nucleolar injury and deformity of root tip cells. The methylation rate of 18SrDNA also changed by NiCl 2 added. Normal 0 21 false false false ES-CL X-NONE X-NONE /* Style Definitions */ table.MsoNormalTable {mso-style-name:Tabla normal; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:Calibri,sans-serif; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-ansi-language:ES-CL; mso-fareast-language:EN-US;}
Highlights
BackgroundNickel (Ni) has been widely applied in industrial and agricultural production and has become a kind of environmental pollution
Since the 20th century, the productive industry has been developing rapidly, and the heavy metals have begun to become a major source of environmental pollutants
The plant nucleolus variation and 18S rDNA methylation may be used as an examination indicator for Ni pollution in soil or plant
Summary
Nickel (Ni) has been widely applied in industrial and agricultural production and has become a kind of environmental pollution. The effect of nickel chloride (NiCl2) with different concentrations on Arabidopsis genomic stability and DNA methylation has been demonstrated. The nucleolus variation and 18S rDNA methylation after NiCl2 treatment have been analyzed. Results: The results are as follows: (1) The NiCl2 could result in heritable genomic methylation variations. (2) NiCl2 brought deformity and damage to nucleolar structure in Arabidopsis root tip cells, and the damage was positively correlated with the NiCl2 concentration. The plant nucleolus variation and 18S rDNA methylation may be used as an examination indicator for Ni pollution in soil or plant. Conclusions: NiCl2 application caused variation of DNA methylation of the Arabidopsis genomic and offspring's. NiCl2 resulted in nucleolar injury and deformity of root tip cells. The methylation rate of 18S rDNA changed by adding NiCl2
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